A method for the production of transgenic plants using...

Details A method for the production of transgenic plants using... A method for the production of transgenic plants using...

A - Human Necessities

01

H

A01H 1/06 (2006.01)

Patent

CA 2321044

This application provides a method for transformation of cells of plant tissues and the regeneration of these cells and tissues into mature transgenic plants. The apical meristematic region of a plant seedling are isolated, then treated to slow the metabolic activity of the isolated tissue. The tissue is then exposed to a transforming agent such as a DNA plasmid or an Agrobacterium carrying a plasmid vector which contains a DNA encoding a gene of interest. After an appropriate incubation period, the treatment is reversed, allowing the metabolic activity of the apical meristematic tissues to return to normal. The method provides a larger population of receptive cells for exposure to the transforming agents, thereby providing higher probabilities of isolating transformed tissues which can be regenerated into transformed plants.

L'invention concerne un procédé de transformation de cellules de tissus végétaux et la régénération de ces cellules et tissus en plantes transgéniques adultes. Des méristèmes apicaux d'une jeune plante sont isolés, puis traités de façon à ralentir l'activité métabolique du tissu isolé. Le tissu est ensuite exposé à un agent transformant tel qu'un plasmide d'ADN ou une agrobactérie portant un vecteur plasmidique qui contient un ADN codant pour un gène intéressant. Après une période d'incubation appropriée, le traitement est inversé, ce qui permet aux tissus méristématiques apicaux de retrouver une activité métabolique normale. Le procédé prévoit une grande population de cellules réceptives en vue de l'exposition aux agents transformants, ce qui permet d'accroître les probabilités d'isoler des tissus transformés pouvant être régénérés en plantes transformées.

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