A method for transfection of rna using electrical pulses

Details A method for transfection of rna using electrical pulses A method for transfection of rna using electrical pulses

C - Chemistry, Metallurgy

12

N

C12N 15/87 (2006.01) A61K 35/12 (2006.01) A61K 48/00 (2006.01) A61P 35/00 (2006.01) C12N 13/00 (2006.01) C12N 15/64 (2006.01)

Patent

CA 2446036

The present invention relates to a method of inserting RNA into cells. In this method, cells are transfected with RNA using electroporation in order to achieve high transfection efficiency. The method is useful, inter alia, in providing cells to be used in cell-based therapies, e.g. in preparing cells useful as anti-cancer vaccines. Preferably, the RNA has a 5' cap and a 3' poly (A) tail.

La présente invention porte sur un procédé visant à introduire l'ARN dans des cellules. Selon ce procédé, ces cellules sont transfectées avec l'ARN par électroporation de sorte que la transfection soit extrêmement efficace. Ce procédé est utile, entre autre, dans la production de cellules destinées à être utilisées dans des thérapies cellulaires, par exemple, dans la préparation de cellules utiles dans les vaccins anticancéreux. De préférence, l'ARN a une coiffe 5' et une queue 3' poly (A).

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