C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/81 (2006.01) C11D 3/386 (2006.01) C12N 9/42 (2006.01) C12N 15/56 (2006.01) C12N 15/64 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2124939
2124939 9311249 PCTABS00022 Method of screening for a DNA sequence coding for a protein of interest, the method comprising a) cloning, in suitable vectors, a DNA library from an organism suspected of producing one or more proteins of interest; b) transforming suitable yeast host cells with said vectors; c) culturing the host cells under suitable conditions to express any protein of interest encoded by a clone in the DNA library, and d) screening for positive clones by determining any activity of a protein expressed in step (c). An enzyme which exhibits cellulase activity and has been isolated from DNA library of Humicola insolens. The enzyme has a cellulose binding domain and exhibits endocellulase activity in the presence of linear alkyl benzene sulfonate.
Dalboge Henrik
Heldt-Hansen Hans P.
Rasmussen Grethe
Mccarthy Tetrault Llp
Novo Nordisk A/s
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