C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/02 (2006.01)
Patent
CA 2595638
A method for cryopreserving cells entails the liposomal delivery of intracellular sugar(s), such as trehalose, sucrose, raffinose, stachyose, and combinations thereof, into cells and tissues, such as red blood cells, for enhancing post-thaw viability. This method enables rapid and easy delivery of protective molecules into cells which thus greatly simplifies the preparation of cells for cryopreservation. Furthermore, as much lower concentrations of intracellular protectant are used, the method allows red blood cells containing the liposomally-delivered intracellular sugar to be transfused into a patient immediately following the thaw without having to first remove any of the cryoprotectant sugar.
L'invention concerne une méthode de cryoconservation de cellules impliquant l'apport liposomal de sucre(s) intracellulaire(s), tel que le tréhalose, le sucrose, le raffinose, la stachyose et des combinaisons de ces derniers, dans des cellules et des tissus, tels que des globules rouges, afin d'améliorer la viabilité post-décongélation. Ladite méthode permet l'apport rapide et aisé de molécules protectrices dans des cellules, ce qui simplifie ainsi considérablement la préparation de cellules pour la cryoconservation. De plus, étant donné que des concentrations beaucoup plus faibles de protecteur intracellulaire sont utilisées, la méthode permet la transfusion des globules rouges contenant le sucre intracellulaire apporté par voie liposomale à un patient immédiatement après la décongélation sans avoir à tout d'abord éliminer un quelconque sucre cryoprotecteur.
Acker Jason P.
Gyongyossy-Issa Maria
Canadian Blood Services
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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