A method of detecting and discriminating between nucleic...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2115342

The present invention is directed to a method for detecting the presence or absence of any specific target nucleic acid se- quence contained in a sample. The target sequence can be present in the sample in a relatively pure form or as a component of a member of a mixture of different nucleic acids. The method of the invention utilizes a novel primer design. The sequence of the novel primer is composed of two portions, the 3' portion is a primer specific for the desired nucleic acid sequence and the 5' por- tion is complementary to preselected nucleic acid sequence. Extension of the 3' portion of the primer with a labeled deoxynucleo- sides triphosphate yields a labeled extension product if, but only if, the template includes the target sequence. The labeled exten- sion product is detected by hybridization of the 5' portion to the preselected sequence. The preselected sequence is preferably bound to a solid support as one member of a grid having a group of sequences.

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