C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 14/61 (2006.01) C07K 1/113 (2006.01) G01N 33/68 (2006.01) G01N 33/74 (2006.01)
Patent
CA 2160663
A method of detecting and treating a polypeptide. A hydrophobic derivative of a growth hormone may be detected by hydrophobic interaction chromatography and elution using a gradient of ammonium sulphate followed by peptide mapping. The derivative may be treated with a mercapto compound for converting the derivative into the native form of the growth hormone. Preferably, the growth hormone is human growth hormone, and the mercapto compound is cysteine in a concentration of 1-2 mM.
Christensen Thorkild
Sorensen Hans Holmegaard
Mccarthy Tetrault Llp
Novo Nordisk A/s
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