A method of determining ace2-activity

Details A method of determining ace2-activity A method of determining ace2-activity

G - Physics

01

N

G01N 33/573 (2006.01)

Patent

CA 2666175

The present invention provides a method for determining ACE2 activity, said method comprising the following steps: in order to provide ACE2-binding units which are immobilized on a solid carrier and are specific to a part of the ACE2 that is not involved in the catalytic activity of ACE2, the immobilized ACE2-binding units are brought into contact with a sample which potentially contains the ACE2, wherein the ACE2 is bound by the ACE2-binding unit, the non-binding parts of the sample are removed from the ACE2 that is bound to the ACE2-binding units, an ACE2 substrate which is converted by the ACE2 activity is added and the conversion provides a signal, and the change in the signal is measured in a particular period of time, wherein the change can be correlated with the ACE2 activity.

La présente invention concerne un procédé de détermination de l'activité de l'ECA2, comprenant les étapes suivantes consistant à : obtenir des motifs de liaison à l'ECA2 immobilisés sur un support solide et spécifiques à une partie de l'ECA2 qui n'est pas impliquée dans l'activité catalytique de l'ECA2 ; mettre en contact les motifs de liaison à l'ECA2 immobilisés avec une sonde contenant potentiellement l'ECA2, l'ECA2 étant liée par le motif de liaison à l'ECA2 ; éliminer les parties de la sonde qui ne se lient pas de l'ECA2 liée aux motifs de liaison à l'ECA2 ; ajouter un substrat de l'ECA2 qui est mis en réaction par l'activité de l'ECA2, cette réaction produisant un signal ; mesurer la variation du signal dans un intervalle de temps défini, cette variation pouvant être corrélée avec l'activité de l'ECA2.

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