A method of dna amplification

Details A method of dna amplification A method of dna amplification

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2077135

ABSTRACT A METHOD OF DNA AMPLIFICATION This invention relates to a method of amplifying template DNA by the PCR reaction wherein (a) a single oligonucleotide is contacted with said template DNA whereby said oligonucleotide randomly anneals to a single strand of said template DNA and DNA sequences complementary to the said single strand are synthesized using said oligonucleotide as a primer and said oligonucleotide also randomly anneals to the complementary strands whereby DNA sequences corresponding to the template DNA are synthesised so that PCR amplification yields synthetic DNA sequences which each incorporate said oligonucleotide sequence at the 5' end, and (b) said synthesised DNA sequences then being subjected to PCR amplification under conditions of stringency higher than in step (a), It is possible by means of the method of this present invention to amplify trace quantities of DNA fragments of unknown sequence simply and efficiently, which is useful in the construction of DNA libraries of chromosome specific regions and the development of probes for their physical mapping

Affiliated with

Also associated with

Rating

A method of dna amplification does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with A method of dna amplification, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and A method of dna amplification will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1465849