A method of enhancing recombinant protein production

Details A method of enhancing recombinant protein production A method of enhancing recombinant protein production

C - Chemistry, Metallurgy

12

N

C12N 15/79 (2006.01) C07K 14/495 (2006.01) C07K 14/51 (2006.01) C12N 9/64 (2006.01) C12N 15/67 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2688232

The present invention provides a method of enhanced protein production that comprises the step of expressing a recombinant gene encoding the protein in eukaryotic cells under conditions in which cleavage of the pro-domain of the protein is inhibited or eliminated. Generally the method of the present invention includes the step of inhibiting or altering the cleavage of a pro-domain of a recombinant protein of interest in order to increase the amount of recombinant protein secreted from a eukaryotic cell. Recombinant proteins that can be prepared using the method of this invention include members of the transforming growth factor-ÿ (TGF-ÿ) superfamily, such as bone morphogenetic proteins. Also provided are genetically engineered cells and polynucleotides for performing the method of the invention.

La présente invention concerne un procédé de production améliorée d'une protéine qui comprend l'étape consistant à exprimer un gène recombinant codant pour la protéine dans des cellules eucaryotes dans des conditions dans lesquelles le clivage du pro-domaine de la protéine est inhibé ou éliminé. De manière générale, le procédé de la présente invention comprend l'étape consistant à inhiber ou à altérer le clivage d'un pro-domaine d'une protéine recombinante intéressante afin d'augmenter la quantité de protéine recombinante sécrétée par une cellule eucaryote. Les protéines recombinantes qui peuvent être préparées en utilisant le procédé de cette invention comprennent des éléments de la superfamille du facteur de croissance transformant .gamma. (TGF-.gamma.), telles que les protéines morphogénétiques osseuses. L'invention concerne également des cellules et des polynucléotides génétiquement modifiés pour effectuer le procédé selon l'invention.

Affiliated with

Also associated with

Rating

A method of enhancing recombinant protein production does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with A method of enhancing recombinant protein production, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and A method of enhancing recombinant protein production will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1849103