C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) C07K 14/415 (2006.01) C07K 14/475 (2006.01) C07K 19/00 (2006.01) C12N 15/18 (2006.01) C12N 15/29 (2006.01) C12N 15/62 (2006.01) C12P 21/00 (2006.01)
Patent
CA 2587092
The present invention provided a method of using cereal non-storage protein as fusion carrier to highly express small peptides in host endosperm cells, comprising the steps of providing an endosperm-specific promoter and a DNA leading sequence encoding an endosperm-specific signal peptide; providing the gene of a non- storage protein as fusion carrier and an target gene; constructing a expression vector containing the promoter and DNA leading sequence, the gene of the fusion carrier, and a target gene; and expressing the expression vector in a host endosperm cell. Also provided in the invention are a vector constructed there from and the use thereof.
Xie Tingting
Yang Daichang
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Healthgen Biotechnology Inc. In Wuhan
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