A method of identifying a nonparaffinophilic microorganism...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/04 (2006.01) C12M 1/16 (2006.01)

Patent

CA 2237165

A method of determining the presence or absence of a nonparaffinophilic microorganism in a specimen taken from a patient is disclosed. The method includes providing a receptacle containing an aqueous solution (13) and adjusting the solution to mimic the in vivo clinical conditions of the patient. The method further includes inoculating the solution with the specimen and then placing in the receptacle a slide (18) coated with a carbon source to bait the nonparaffinophilic microorganism. The slide (18) is then analyzed after exposure to the specimen to determine the presence or absence of the nonparaffinophilic microorganism. An associated apparatus is also disclosed. The front elevational view of a test tube (12) holding a slide coated with a carbon source in an aqueous solution (13) inoculated with a specimen.

Procédé permettant de déterminer la présence ou l'absence d'un micro-organisme nonparaffinophile dans un échantillon prélevé sur un patient, qui consiste à verser une solution aqueuse (13) dans un récipient et à adapter ladite solution afin qu'elle imite les conditions cliniques in vivo du patient. Ledit procédé consiste en outre à inoculer le prélèvement à ladite solution et à placer ensuite dans le récipient une lame (18) couverte d'une source de carbone afin d'attirer le micro-organisme nonparaffinophile. Ladite lame (18) est ensuite analysée après exposition au prélèvement pour déterminer la présence ou l'absence dudit micro-organisme nonparaffinophile. Un appareil associé est également décrit. La vue de face d'un tube d'analyse (12) contenant une lame couverte d'une source de carbone dans une solution aqueuse (13) à laquelle on a inoculé le prélèvement est également présentée.

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