A method of identifying ligands and antagonists of ligands

C - Chemistry – Metallurgy – 12 – Q

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Details

C12Q 1/68 (2006.01) C12N 5/10 (2006.01) C12Q 1/02 (2006.01) C12Q 1/34 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2120518

The present invention relates, in general, to a method of identifying ligands and antagonists of ligands. In particular, the present invention relates to a method of identifying ligands and antagonists of ligands which bind to cloned Gs- or G1-coupled receptors. The present invention also relates to a cell that comprises a recumbinant cyclic AMP sensitive reporter construct.

La présente invention porte, de façon générale, sur une méthode d'identification de ligands et d'antagonistes de ligands. La présente invention décrit en particulier une méthode d'identification de ligands et d'antagonistes de ligands qui se lient à des récepteurs clonés couplés à Gs ou G1. La présente invention porte également sur une cellule qui comprend un construit marqueur recombinant sensible à l'AMP cyclique.

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