A method of permanent fluorescent assay

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/28 (2006.01) G01N 33/52 (2006.01) C07C 235/34 (2006.01)

Patent

CA 2332560

A method for performing a peroxidase-based assay by reacting a peroxidase enzyme with a soluble organic p-hydroxyphenyl-containing compound having formula (I) wherein Q is a linear or branched 1-12 heteroatom alkyl wherein the heteroatoms are selected from C, N, O and S, wherein the bonds connecting the heteroatom alkyl chain are single or double, wherein any carbon atom in the heteroatom alkyl chain optionally includes a substituent selected from -OH, -COOH, -NH2, and -SH and wherein R is selected from -OH, -COOH, -NH2 and -CH3; and converting the soluble organic p-hydroxyphenyl-containing compound into a stable insoluble product which becomes highly fluorescent after illumination, upon excitation.

L'invention concerne un procédé permettant d'effectuer un dosage à base de peroxydase en faisant réagir une peroxydase avec un composé soluble organique contenant du p-hydroxyphényle représenté par la formule (I), dans laquelle Q représente un alkyle linéaire ou ramifié à 1-12 hétéroatomes, lesquels sont choisis dans le groupe constitué de C, N, O et S, où les liaisons reliant la chaîne alkyle à hétéroatomes comprennent éventuellement un substituant choisi dans le groupe constitué de -OH, -COOH, -NH¿2? et -SH et où R est choisi dans le groupe constitué de -OH, -COOH, -NH¿2? et -CH¿3?; et en convertissant le composé soluble organique contenant du p-hydroxyphényle en un produit insoluble stable qui devient fortement fluorescent après avoir été excité et illuminé.

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