A method of preparing cross-linked enzyme aggregates

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 9/96 (2006.01) C12N 9/20 (2006.01) C12N 11/02 (2006.01) C12P 7/00 (2006.01)

Patent

CA 2436087

The invention relates to a method of preparing cross-linked enzyme aggregates (CLEAs) comprising the steps of a) precipitating a dissolved enzyme present in a liquid medium; b) cross-linking with a cross-linker, and c) optionally, washing the cross-linked enzyme aggregate. According to the invention at least one treatment is performed chosen from the group consisting of I) performing step a) in the presence of a compound selected from the group consisting of i) a crown ether, and ii) a surfactant, and II) performing step c) with a second liquid differing in organic composition from the liquid.

La présente invention concerne une technique de préparation d'agrégats d'enzymes réticulées qui consiste: (a) à précipiter une enzyme dissoute présente dans un milieu liquide, (b) à effectuer une réticulation avec un agent de réticulation et (c), éventuellement, à laver cet agrégat d'enzymes réticulées. Selon cette invention, on effectue au moins un traitement choisi parmi les étapes suivantes: (I) traitement de l'étape (a) en présence d'un composé sélectionné dans le groupe constitué de (i) éther couronne et (ii) d'un tensioactif, et (II) traitement de l'étape (c) avec un second liquide différent quant à sa composition organique.

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