A method of preparing ligands for hydrophobic interaction...

Details A method of preparing ligands for hydrophobic interaction... A method of preparing ligands for hydrophobic interaction...

B - Operations, Transporting

01

D

B01D 15/08 (2006.01) B01J 20/22 (2006.01) B01J 20/30 (2006.01)

Patent

CA 2517757

The invention is a method of preparing multimodal ligands for hydrophobic interaction chromatography (HIC), which comprises providing a cyclic scaffold comprising a thiol, an amine and a carbonyl group; derivatisation of the nitrogen with a reagent to introduce a primary interaction; and aminolysis of the resulting derivative, whereby a secondary interaction is introduced next to the carbonyl; wherein at least one of the primary interaction and the secondary interaction comprises a hydrophobic group and wherein non of said interactions comprises charged groups, i.e. ion exchange ligands. The invention also encompasses a separation medium comprising such multimodal ligands immobilised to a base matrix.

La présente invention concerne un procédé de préparation de ligands multimodaux destinés à la chromatographie d'interaction hydrophobe (CIH) qui consiste à utiliser un squelette cyclique comprenant un thiol, une amine et un groupe carbonyle; à former un dérivé de l'azote avec un réactif en vue de produire une interaction primaire; et à pratiquer l'aminolyse du dérivé résultant, ceci permettant d'introduire une interaction secondaire suite au carbonyle; de sorte qu'au moins une des interactions primaire et secondaire comprenne un groupe hydrophobe et qu'aucune desdites interactions ne comprenne de groupes chargés, c'est-à-dire de ligands échangeurs d'ions. Cette invention se rapporte également à un milieu de séparation comprenant de tels ligands multimodaux immobilisés sur une matrice de base.

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