A method of producing insulinotropic glp-1 (7-36)...

Details A method of producing insulinotropic glp-1 (7-36)... A method of producing insulinotropic glp-1 (7-36)...

C - Chemistry, Metallurgy

12

P

C12P 21/02 (2006.01) C07K 14/605 (2006.01) C07K 19/00 (2006.01) C12N 15/16 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2454264

The present invention relates to a method of producing glucagon-like peptide 1(GLP-1)7-36 and an GLP-1 analogue by consecutive genes, and peptide produced by said method. In the present invention, 1-32 GLP-1(7-36) genes or GLP-1 analogue genes can be expressed in tandem, subsequently, the target peptide can be obtained by several steps including fusion protein lysis, isolation and purification. The present invention can decrease production cost dramatically, also can produce GLP-1(7-36) and GLP-1 analogue in large scale.

La présente invention concerne une méthode de production de peptide 1 de type glucagon (GLP-1) 7-36 et d'un analogue de GLP-1 par gènes consécutifs, ainsi qu'un peptide produit par ladite méthode. Dans la présente invention, des gènes 1-32 GLP-1 (7-36) ou des gènes d'analogue de GLP-1 peuvent être exprimés en tandem, ensuite, le peptide cible peut être obtenu par plusieurs étapes comprenant la lyse, l'isolement et la purification de protéines de fusion. La présente invention permet de réduire considérablement le coût de production, elle permet également de produire le GLP-1 (7-36) ainsi qu'un analogue de GLP-1 à grande échelle.

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