C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/21 (2006.01) C12N 9/10 (2006.01) C12N 15/54 (2006.01)
Patent
CA 2387823
A process for the secretory production of transglutaminase by using a microorganism. A process for the mass production of transglutaminase characterized by comprising culturing a bacterium belonging to the genus Streptomyces which has an expression plasmid carrying a transglutaminase gene originating in actinomycetes and the inherent (intact) promoter thereof, thus making the bacterium to secret protransglutaminase in the early stage to the medium stage of the culture, and then cutting off and removing the pro- structure with Streptomyces-origin protease, etc. in the latter stage of the culture to thereby give matured (active) transglutaminase.
La présente invention concerne un procédé de production sécrétoire de transglutaminase mettant en oeuvre un micro-organisme. Ce procédé de production en série de transglutaminase est caractérisé en ce qu'il comporte: la culture d'une bactérie appartenant au genre Streptomyces qui présente une expression plasmidique portant le gène de la transglutaminase dérivé des actinomycètes et le promoteur inné (intact) de ceux-ci, permettant ainsi à la bactérie de sécréter de la protransglutaminase à un stade précoce au milieu de culture, et ensuite la coupe et l'extraction de la pro-structure avec la protéase dérivée des Streptomyces, et ainsi de suite, dans le stade avancé de la culture afin d'obtenir de la transglutaminase mûrie (active).
Momose Haruo
Taguchi Seiichi
Ajinomoto Co. Inc.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
LandOfFree
A method of producing microbial transglutaminase does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with A method of producing microbial transglutaminase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and A method of producing microbial transglutaminase will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-2015262