A method of purifying factor viii/vwf-complex by means of...

C - Chemistry – Metallurgy – 07 – K

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C07K 14/755 (2006.01) A61K 38/36 (2006.01) A61K 38/37 (2006.01) A61P 7/04 (2006.01) C07K 1/18 (2006.01) C07K 14/745 (2006.01)

Patent

CA 2282841

There is disclosed a method of recovering factor VIII/vWF-complex which is characterized in that factor VIII/vWF-complex from a protein solution is bound to a cation exchanger and is recovered by step-wise elution of factor VIII/vWF-complex, which particularly contains high-molecular vWF multimers, as well as a factor VIII/vWF-complex obtainable by means of cation exchange chromatography.

L'invention concerne un procédé permettant d'obtenir un complexe facteur VIII/vWF (vWF étant le facteur von Willebrand), caractérisé en ce que le complexe facteur VIII/vWF est obtenu à partir d'une solution protéique liée à un échangeur de cations. Le complexe facteur VIII/vWF, contenant notamment des multimères du vWF de poids moléculaire élevé, est obtenu par élution graduelle. L'invention concerne également un complexe facteur VIII/vWF pouvant être obtenu par chromatographie sur échangeur de cations.

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