C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12N 15/75 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2540985
The invention describes a method of screening for protein secreting recombinant host cells comprising screening for promoter activity of a stress inducible promoter. The method can be used for rapid identification of actively secreting transformants and can be used to screen recombinant libraries for transformants secreting proteins.
L'invention concerne une méthode de criblage destinée à identifier une protéine sécrétant des cellules hôtes recombinantes. La méthode consiste à effectuer un criblage destiné à identifier une activité promotrice d'un promoteur d'induction de stress. La méthode peut être mise en oeuvre pour identifier rapidement des transformants à sécrétion active et pour cribler des bibliothèques recombinantes en vue d'identifier des protéines sécrétant des transformants.
Dimock Stratton Llp
Novozymes A/s
LandOfFree
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