A method of separating a peptidoglycan recognition protein...

C - Chemistry – Metallurgy – 07 – K

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C07K 1/14 (2006.01) C07K 1/16 (2006.01)

Patent

CA 2636194

Provided is a method of separating a peptidoglycan-recognition protein from the hemolymph of Tenebrio molitor larvae, the method including: (a) preparing an affinity chromatography column by packing a column with a resin bound with at least one synthetic peptidoglycan; (b) applying the hemolymph of Tenebrio molitor larvae to the affinity chromatography column; (c) applying a mobile phase to the affinity chromatography column to obtain a peptidoglycan- recognition protein-containing solution eluted from the affinity chromatography column; and (d) purifying a peptidoglycan-recognition protein from the eluted solution.

L'invention porte sur un procédé de séparation d'une protéine de reconnaissance du peptidoglycane d'avec une hémolymphe de larve de Tenebrio molitor. Ledit procédé comporte les étapes suivantes: (a) préparation d'une colonne de chromatographie par affinité en remplissant une colonne d'une résine liée à au moins un peptidoglycane de synthèse; (b) application de l'hémolymphe de larve de Tenebrio molitor à la colonne de chromatographie par affinité; (c) application d'une phase mobile à la colonne de chromatographie par affinité pour obtenir une solution contenant une protéine de reconnaissance du peptidoglycane éluée par la colonne de chromatographie par affinité; et (d) purification de la protéine de reconnaissance du peptidoglycane de la solution éluée.

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