A method of sequencing proteins by epitope ordering and...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 16/44 (2006.01) C07H 21/00 (2006.01) C07K 16/00 (2006.01) C07K 16/18 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/563 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2197493

Methods of identifying an amino acid sequence of a protein and characterizing post-translational events occurring on the protein through epitope ordering followed by restriction mapping are provided. Modified antibodies containing restriction enzyme sites which are capable of recognizing and binding to amino acids as an epitope or post-translationally modified epitopes are also provided.

La présente invention concerne des procédés d'identification d'une séquence d'acides aminés d'une protéine et de caractérisation des événements post-traduction survenant sur la protéine au cours du classement d'épitopes suivant une cartographie des sites de restriction. L'invention concerne également des anticorps modifiés contenant des sites d'enzymes de restriction, et qui sont capables de reconnaître des acides aminés et de se lier à eux en tant qu'épitope ou en tant qu'épitopes modifiés après traduction.

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