A method of stabilizing recombinant hepatitis b virus...

Details A method of stabilizing recombinant hepatitis b virus... A method of stabilizing recombinant hepatitis b virus...

C - Chemistry, Metallurgy

07

K

C07K 14/02 (2006.01) C07K 1/36 (2006.01)

Patent

CA 2062460

12/JWW19 17881IA TITLE OF THE INVENTION A METHOD OF STABILIZING RECOMBINANT HEPATITIS B VIRUS SURFACE PROTEINS FROM RECOMBINANT HOST CELLS ABSTRACT OF THE DISCLOSURE Recombinant hepatitis B virus surface proteins produced in recombinant host cells are rapidly and efficiently purified from heated whole cells at neutral pH. The whole cells are heat treated, cooled and in the case of high pH extract, the pH is reduced. The surface proteins are then adsorbed onto wide pore silica followed by elution and concentration. This method eliminates the requisite introduction of protease inhibitors, stabilizes the surface protein and improves product yield.

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