A - Human Necessities – 61 – K
Patent
A - Human Necessities
61
K
A61K 48/00 (2006.01) A61K 38/44 (2006.01) C12M 3/00 (2006.01) C12N 5/00 (2006.01) C12N 5/02 (2006.01)
Patent
CA 2340086
In accordance with the present invention, there are provided methods of transducing cells comprising providing a flexible closed culture container having cells therein and contacting said cells with a viral-vector in the presence of a multi-fonctional chemical moiety. Also provided are methods of delivering a functional protein to a subject in need thereof, comprising transducing mammalian cells according to the invention method and introducing said cells into a subject in need thereof. Also provided are cell-culture systems for transducing cells, comprising a flexible closed culture container and a multi-functional chemical moiety therein.
L'invention concerne des procédés relatifs à la transduction de cellules qui consistent à utiliser un contenant à culture clos et flexible renfermant des cellules, et à mettre ces cellules en contact avec un vecteur viral en présence d'une fraction chimique multivalente. L'invention concerne également des procédés relatifs à l'administration d'une protéine fonctionnelle à un sujet ayant besoin de cette protéine, qui consistent à assurer la transduction de cellules mammaliennes selon les procédés de transduction susmentionnés et à introduire lesdites cellules dans l'organisme d'un sujet ayant besoin de ces cellules. L'invention concerne en outre des systèmes de culture cellulaire permettant d'assurer la transduction de cellules, qui comprennent un contenant à culture clos et flexible dans lequel on utilise une fraction chimique multivalente.
Malech Harry L.
Gowling Lafleur Henderson Llp
The United States Of America Represented By Department Of Health And Hu Man Services
LandOfFree
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