A method to produce succinic acid from raw hydrolysates

C - Chemistry – Metallurgy – 12 – P

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C12P 7/00 (2006.01) C12N 1/20 (2006.01) C12N 15/00 (2006.01) C12N 15/74 (2006.01) C12P 7/46 (2006.01) C12P 7/54 (2006.01)

Patent

CA 2505423

A method for producing succinic acid from industrial-grade hydrolysates is provided, comprising supplying an organism that contains mutations for the genes ptsG, pflB, and ldhA, allowing said organism to accumulate biomass, and allowing said organism to metabolize the hydrolysate. Also provided is a bacteria mutant characterized in that it produces succinic acid from substrate contained in industrial-grade hydro-lysate in a ratio of between 0.6:1 and 1.3:1 succinic acid to substrate.

Cette invention concerne un procédé permettant de produire de l'acide succinique à partir d'hydrolysats de qualité industrielle et consistant à utiliser un organisme contenant des mutations pour les gènes ptsG, pflB et ldhA, à permettre à cet organisme de stocker de la biomasse et à permettre à cet organisme de métaboliser l'hydrolysat. Cette invention concerne également une bactérie mutante qui se caractérise en ce qu'elle produit de l'acide succinique à partir du substrat contenu dans l'hydrolysat de qualité industrielle selon un rapport acide succinique/substrat compris entre 0,6:1 et 1,3:1.

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