A micro-volume liquid ejection system

G - Physics – 01 – N

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Details

G01N 35/10 (2006.01) B05B 9/04 (2006.01)

Patent

CA 2585260

The present invention relates to a micro-volume liquid ejection system, including an air pressure module, a micro-ejection unit which is connected with the air pressure module by means of pipes, and a control circuit which is connected with the air pressure module and the micro-ejection unit respectively. In the present invention, due to air is used as the pressure medium, on one hand, as the sample does not contact with the pressure regulating module, the efficiency of cleaning process is improved, on the other hand, the volume of the sample needed in the sample ejecting process is only equal to the cavity-dimension of the micro-ejection unit by not need to fill the whole pipe with liquid. During the sample ejecting process, it is not need to regulate the pressure. After the sample ejecting is finished, the sample can be put into its original place, so as to greatly save the sample. The present invention is connected with the manipulator, so as to sample from the sample plate, ej ect sample and clean the pipes automatically, and it can also dispense a multiplicity of samples to micro-array substrate conveniently. The present invention can be used for transferring or dispensing the micro~volume liquid of nL grade and ~L grade widely, and all kinds of the micro-volume liquid including biological liquid.

La présente invention porte sur un système d~éjection liquide en micro volume, englobant un module de pression d~air, une unité de micro éjection connectée au module de pression d~air au moyen de tuyaux, et un circuit de commande connecté au module de pression d~air et à l~unité de micro éjection respectivement. Dans la présente invention, compte tenu du fait que l~air sert de support de pression, d~une part, dans la mesure où l~échantillon ne touche pas le module de régulation de pression, l~efficacité du cycle de nettoyage est augmentée, et d'autre part, le volume de l~échantillon nécessaire dans le cycle d~éjection d~échantillon est seulement égal à la dimension de cavité de l~unité de micro éjection et il n'est nécessaire remplir de liquide la totalité du tuyau. Pendant le cycle d~éjection d~échantillon, il n~est pas nécessaire de réguler la pression. Une fois l~éjection de l~échantillon terminée, on peut ramener l~échantillon dans son emplacement d~origine pour largement économiser sur l~échantillon. Le système de la présente invention est connecté au manipulateur, pour prélever un échantillon dans la plaque, pour éjecter l~échantillon et nettoyer les tuyaux automatiquement, et il permet également de dispenser une multiplicité d~échantillons au substrat de micro matrice de manière commode. La présente invention peut servir au transfert ou à l~administration de liquide en micro ­volume de nuance nL et de nuance µL à grande échelle, et de toutes sortes de liquide en micro volume englobant un liquide biologique.

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