C - Chemistry – Metallurgy – 40 – B
Patent
C - Chemistry, Metallurgy
40
B
C40B 60/02 (2006.01) C12Q 1/68 (2006.01) C40B 60/08 (2006.01) G01N 33/50 (2006.01) G01N 33/52 (2006.01) G01N 33/543 (2006.01)
Patent
CA 2568904
Glass as a substrate for fluorescence-based assays is expensive. Disclosed is a poly(cyclic olefin) alternative to glass that is of comparable sensitivity. More specifically, disclosed is a method of making a microdevice for the immobilization of biomolecules for the purpose of carrying out a fluorescence-based assay, said method comprising providing a substrate comprising a poly(cyclic olefin); protecting the substrate from ultraviolet (UV) light; and subjecting a surface of said UV-protected substrate to ozone oxidation to activate said surface; wherein a content of intrinsic fluorophores on the surface of the substrate remains substantially unchanged after the ozone oxidation. Also disclosed is a microdevice for the immobilization of biomolecules for the purpose of carrying out a fluorescence-based assay, said microdevice comprising a body comprising a poly(cyclic olefin), said body having an ozone-activated surface substantially free of intrinsic fluorophores; wherein said activated surface is substantially free of intrinsic fluorescence during a fluorescence-based assay of said biomolecules bound to said activated surface.
Diaz-Quijada Gerardo A.
Veres Teodor
Marks & Clerk
National Research Council Of Canada
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