G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 27/26 (2006.01) G01N 27/327 (2006.01)
Patent
CA 2349595
Single-channel thin film devices and methods for using the same are provided. The subject devices comprise cis and trans chambers (1 and 2) connected by an electrical communication means (3). At the cis end of the electrical communication means is a horizontal conical aperture (4) sealed with a thin film that includes a single nanopore or channel. The devices further include a means for applying an electric field between the cis and trans chambers. The subject devices find use in applications in which the ionic current through a nanopore or channel is monitored, where such applications include the characterization of naturally occurring ion channels, the characterization of polymeric compounds, and the like.
L'invention concerne des dispositifs à films minces à un seul canal et des procédés d'utilisation de ces derniers. Ces dispositifs comprennent des chambres cis et trans (1 et 2) reliées par un moyen de communication électrique (3). Au niveau de l'extrémité cis du moyen de communication électrique se trouve une ouverture conique horizontale (4) fermée avec un film mince qui comprend un seul nanopore ou un canal. Ces dispositifs comprennent, en outre, un moyen pour appliquer un champ électrique entre les chambres cis et trans. Ces dispositifs présentent une grande utilité dans des applications dans lesquelles le courant ionique dans un nanopore ou un canal est surveillé, ou dans des applications qui consistent à caractériser les canaux ioniques naturels, à caractériser des composés polymères, etc.
Akeson Mark A.
Branton Daniel
Deamer David W.
President And Fellows Of Harvard College
Smart & Biggar
The Regents Of The University Of California
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