C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/19 (2006.01) C12N 15/52 (2006.01) C12P 7/06 (2006.01) C12P 19/02 (2006.01)
Patent
CA 2483997
The present invention relates to a method for producing a L-arabinose utilizing yeast stain for the production ethanol, whereby a yeast strain is modified by introducing and expressing araAgene (L-arabinose isomerase), araB gene (L-ribulokinase D121-N) and araD gene (L-ribulose-5-P 4-epimerase) and carrying additional mutations in its genome or overexpressing a TAL1 (transaldolase) gene, enabling it to consume L-arabinose, to use it as the only carbon source, and to produce ethanol, as well as a method for producing ethanol using such a modified strain.
La présente invention concerne un procédé de fabrication d'une souche de levure faisant intervenir L-arabinose, destinée à la production d'éthanol, une souche de levure étant modifiée par introduction et expression du gène <i>araA </i> (L-arabinose isomérase), du gène <i>araB</i> (L-ribulokinase D?121¿-N) et du gène <i>araD</i> (L-ribulose-5-P 4-épimérase), et mise en oeuvre de mutations supplémentaires dans son génome ou surexpression d'un gène <i>TAL1</i> (transaldolase) de manière que ladite levure consomme L-arabinose. Ainsi, ladite levure peut être employée comme l'unique source de carbone et pour la production d'éthanol. L'invention concerne également un procédé de production d'éthanol faisant intervenir une telle souche modifiée.
Becker Jessica
Boles Eckhard
Forskarpatent I. Syd Ab
Mccarthy Tetrault Llp
Scandinavian Technology Group Ab
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