A molecule detecting system

G - Physics – 01 – N

Patent

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Details

G01N 21/76 (2006.01) C12Q 1/68 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2723076

The invention disclosed here has several key elements: direct probe-on-sensor detection, mesh probe, and mesh reporter. Furthermore, the mesh probe and reporter shall have some physical cross- linking between the polymer backbone(s), either covalently or non-covalently. In combination, this invention enables one to build an ultra-sensitive, low-cost, and highly portable system for molecular detection. Among its many potential applications is a direct detection of nucleic acids in crude lysate in a multiplex format, without the requirements for nucleic acids extraction and amplification. Such an improvement is likely to change the practice of genotyping and gene expression profiling done in a clininic setting. Another application is an ultrasensitive ELIZA assay in a multiplex format.

La présente invention est caractérisée par plusieurs éléments clés : détection directe sonde sur capteur, sonde à mailles et rapporteur à mailles. La sonde à mailles et le rapporteur doivent en outre avoir une certaine réticulation physique avec le(s) squelette(s) polymère(s), soit de manière covalente, soit de manière non covalente. Cette invention permet, en combinaison, de construire un système ultra-sensible, bon marché et très portable de détection moléculaire. Parmi ses nombreuses applications potentielles se trouve une détection directe dacides nucléiques dans un lysat brut en un format multiplex, sans que lextraction et lamplification dacides nucléiques ne soient nécessaires. Une telle amélioration est susceptible de modifier la pratique du génotypage et du profilage de lexpression génique réalisés dans un environnement clinique. Une autre application est un test ELISA ultrasensible en un format multiplex.

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