A monoclonal antibody to a human mdr1 multidrug resistance...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/13 (2006.01) A61K 39/395 (2006.01) A61K 47/48 (2006.01) C07K 1/16 (2006.01) C07K 16/28 (2006.01) C07K 16/42 (2006.01) C12N 5/00 (2006.01) G01N 33/543 (2006.01) G01N 33/577 (2006.01) A61K 38/00 (2006.01) A61K 43/00 (1990.01)

Patent

CA 2132540

Hybridomas (termed "UIC2 hybridoma", ATCC Accession No. HB11207 and "UIC2/A hybridoma", ATCC Accession No. HB11287) producing monoclonal antibody (termed "UIC2 mAb") directed against an extracelluar domain of a cell surface P-glycoprotein antigen associated with multidrug resistance in primate cells was produced by fusing a human myeloma cell with a spleen cell derived from a Balb/c mouse immunized with syngeneic 3T3 fibroblasts previously transfected with the isolated human mdrl cDNA. UIC2 mAb, thus produced, as well as fragments and recombinant derivative thereof, may be used to detect and isolate multidrug resistant primate cells and human mdrl gene products, and to reverse multidrug resistance in primate cells, including cells of multidrug resistant human tumours.

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