A multi-plasmid method for preparing large libraries of...

Details A multi-plasmid method for preparing large libraries of... A multi-plasmid method for preparing large libraries of...

C - Chemistry, Metallurgy

12

N

C12N 15/52 (2006.01) A61K 31/70 (2006.01) A61P 31/00 (2006.01) C07D 309/00 (2006.01) C07D 313/04 (2006.01) C07D 493/10 (2006.01) C07H 17/04 (2006.01) C07H 17/08 (2006.01) C12N 9/10 (2006.01) C12N 15/10 (2006.01) C12N 15/63 (2006.01) C12N 15/90 (2006.01) C12P 17/08 (2006.01) C12P 19/62 (2006.01) C07B 61/00 (2006.01)

Patent

CA 2369633

A multiple-plasmid system for heterologous expression of polyketides facilitates combinatorial biosynthesis. The method can be extended to any modular polyketide synthase (PKS) or non-ribosomal peptide synthase (NRPS) and has the potential to produce thousands of novel natural products, including ones derived from further modification of the PKS or NRPS products by tailoring enzymes.

L'invention concerne un système multiplasmide destiné à l'expression hétérologue des polycétides et qui facilite la biosynthèse combinatoire. Cette méthode peut s'appliquer à n'importe quelle polycétide synthase modulaire (PKS) ou peptide synthase non ribosomique (NRPS), et peut permettre de produire des milliers de nouveaux produits naturels, y compris des produits dérivés des produits PKS ou NRPS modifiés grâce à l'adaptation des enzymes.

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