A multicellular in vitro assay of angiogenesis

Details A multicellular in vitro assay of angiogenesis A multicellular in vitro assay of angiogenesis

C - Chemistry, Metallurgy

12

Q

C12Q 1/02 (2006.01) C12N 5/06 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2301171

A multicellular in vitro assay for modelling the combined stage of angiogenesis namely the proliferation, migration and differentiation stages of cell development, wherein the assay comprises providing a dual culture of endothelial cells together with another cell-type e.g. fibroblast exhibiting interaction therewith to display the combined stages of angiogenesis in vitro.

L'invention porte sur un dosage multicellulaire in vitro destiné à modéliser l'étape combinée de l'angiogenèse, à savoir les phases de prolifération, migration et différenciation du développement cellulaire. Le dosage consiste à produire une double culture de cellules endothéliales avec un autre type de cellules telles que des fibroblastes avec lesquelles elles ont une interaction de façon à présenter les étapes combinées de l'angiogenèse in vitro.

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