A multistage process for the preparation of highly pure...

C - Chemistry – Metallurgy – 07 – C

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C07C 259/04 (2006.01) C07C 259/06 (2006.01) C07C 309/00 (2006.01)

Patent

CA 2392925

The present invention provides a purification process whereby deferoxamine B produced by a microorganism and in mixture with other polyhydroxamates produced by the microorganism may be converted into its mesylate salt substantially free of the other polyhydroxamates and substantially free of chloride ion. The process includes adsorption and desorption of the deferoxamine B on an adsorption resin, direct precipitation of the deferoxamine free base out of the eluent from the adsorption resin, contacting of the deferoxamine B free base with methanesulfonic acid and isolation of the deferoxamine B mesylate salt by precipitation. This process minimizes decomposition of deferoxamine B.

La présente invention concerne un procédé de purification dans lequel de la déferoxamine B produite par un micro-organisme, et en mélange avec d'autres polyhydroxamates produits par ce micro-organisme, peut être convertie en sel méthanesulfonate ne contenant sensiblement pas d'autres polyhydroxamates et d'ion chlorure. Le procédé consiste à adsorber et désorber la déferoxamine B sur une résine d'adsorption, à effectuer une précipitation directe de la déferoxamine base libre de l'éluant sortant de la résine d'adsorption, à mettre en contact la déferoxamine B base libre avec de l'acide méthane sulfonique et à isoler par précipitation le sel de déferoxamine B sous forme de méthanesulfonate. Ce procédé permet de minimiser la décomposition de la déferoxamine B.

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