A mutated subtilisin protease

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/50 (2006.01) C11D 3/386 (2006.01) C11D 7/42 (2006.01) C12N 9/54 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 2062732

Enzymes produced by mutating the genes for a number of subtilisin proteases and expressing the mutated genes in suitable hosts are presented. The mutated enzymes have a changed net electrostatic charge compared to the parent protease at the same pH. The enzymes exhibit improved wash performance in comparison to their wild type parent enzymes. The enzymes are well-suited for use in detergent compositions.

L'invention concerne des enzymes produites par mutation des gènes destinés à un certain nombre de protéases de subtilisine, et par expression des gènes ayant subi une mutation dans des hôtes adaptés. Les enzymes ayant subi une mutation présentent une charge électrostatique nette changée par rapport à la protéase mère au même pH. Les enzymes présentent une capacité de lavage supérieure par rapport à leurs enzymes mère de type sauvage. Les enzymes sont bien adaptées pour être utilisées dans des compositions de détergents.

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