A neural network assisted multi-spectral segmentation system

G - Physics – 06 – T

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G06T 7/00 (2006.01) A61B 5/00 (2006.01) G01N 15/14 (2006.01) G06K 9/00 (2006.01) G06T 1/40 (2006.01) G06T 5/00 (2006.01)

Patent

CA 2232164

A neural network assisted multi-spectral segmentation method and system. According to the invention, three images having different optical bands are acquired for the same micrographic scene of a biological sample. The images are processed and a cellular material map is generated identifying cellular material. The cellular material map is then applied to a neural network. The neural network classifies the cellular material map into nuclear objects and cytoplasmic objects by determining a threshold surface in the 3-dimensional space separating the cytoplasmic and nuclear regions. In another aspect, the neural network comprises a hardware-encoded algorithm in the form of a look-up table.

Procédé et système de segmentation multispectre assistée par réseau neuronal. Selon l'invention, trois images présentant des bandes optiques différentes sont saisies pour la même scène micrographique d'un échantillon biologique. Ces images sont traitées et une carte de matériaux cellulaires est produite, identifiant le matériau cellulaire. La carte de matériaux cellulaire est alors appliquée sur un réseau neuronal. Le réseau neuronal distingue dans la carte de matériaux cellulaires les objets nucléaires et les objets cytoplasmiques, en déterminant une surface seuil, dans l'espace en trois dimensions, séparant les régions cytoplasmique et nucléaire. Selon une autre configuration, le réseau neuronal est équipé d'un algorithme programmé dans le matériel sous la forme d'une table de recherche.

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