A new cephalosporin c acylase

Details A new cephalosporin c acylase A new cephalosporin c acylase

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) C12N 9/80 (2006.01) C12P 35/02 (2006.01)

Patent

CA 2175338

A mutant CC acylase wherein at least one amino acid at the A1a49, Met164, Ser166, Met174, Glu358, Met465, Met506, or Met750 position of the animo acid sequence of the native CC acylase is replaced by a different amino acid, a DNA coding therefor, an expression vector containing the said DNA, a microorganism transformed with the said expression vector, the production of the CC acylase by culturing the said transformant, and use thereof for the production of a compound. The mutant CC acylase of the invention has desirable properties in terms of enzymatic potency, alteration of pH profile, efficiency of processing, and the like.

L'invention concerne une CC (céphalosporine C) acylase mutante dans laquelle au moins un acide aminé en position Ala?49¿, Met?164¿, Ser?166¿, Met?174¿, Glu?358¿, Met?465¿, Met?506¿ ou Met?750¿ de la séquence d'acide aminé de la CC acylase native est remplacé par un acide aminé différent. L'invention concerne aussi l'ADN pouvant la coder, un vecteur d'expression contenant cet ADN, un micro-organisme transformé à l'aide de ce vecteur d'expression, la production de CC acylase par culture dudit transformant et son utilisation pour la production d'un composé. La CC acylase mutante de l'invention présente des propriétés recherchées en matière de puissance enzymatique, de modification du profil de pH, d'efficacité de production, etc.

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