A novel cytoplasmic male sterility system for brassica...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01)

Patent

CA 2578187

The present invention relates to a novel cytoplasmic male sterility (CMS) 126- 1 in Brassica species- examples given here being B. napus and B. juncea and a method for obtaining male sterile lines using the said CMS. The CMS has a distinct mitochondrial DNA specific RFLP signature. The invention also relates to a method for obtaining male sterile lines in Brassica species and restoration of fertility in B. juncea for hybrid seed production. This CMS in B. juncea can be restored by crossing male sterile plants with any B. juncea genotype other than the maintainer genotype. The same B. juncea genotype acts as a maintainer of male sterility after a specified number of backcrosses. This invention also relates to the histological characterization of anther and microspore development in B. juncea.

La présente invention a pour objet un nouveau système de stérilité mâle cytoplasmique (CMS) 126-1 des espèces de Brassicées ; des exemples en sont ici proposés, comme le B. napus et le B. juncea, ainsi qu'un procédé permettant d~obtenir des lignées stériles mâles à l'aide dudit CMS. Ce CMS présente une signature RFLP spécifique d'ADN mitochondrial distinct. Cette invention concerne également un procédé permettant d~obtenir des lignées stériles mâles d~espèces de Brassicées ainsi que la restauration de la fertilité de le B. juncea pour la production de graines hybrides. Ce CMS dans B. juncea peut être restauré par le croisement de plantes stériles mâles avec tout génotype B. juncea autre que le génotype de maintien. Ce même génotype B. juncea agit comme un agent de maintien de la stérilité mâle après un nombre donné de rétrocroisements. Cette invention a également pour objet la caractérisation histologique de l'anthère et le développement de microspores dans le B. juncea.

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