A novel diagnostic marker for splicing variants of genes...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) G01N 33/566 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2235593

Methods are described for detecting the presence or absence of a four amino acid motif (VRXQ) in expressed proteins that arise from aberrant alternative splicing of pre-mRNA in genes associated with normal neurological function. The presence of these variants suggest that mutational events in these genes have occurred. Nucleotide sequences and nitron-exon junctional sequences of examples of this splicing variant are provided.

L'invention concerne des méthodes de détection de la présence ou l'absence d'un motif à quatre acides aminés (VRXQ) dans des protéines exprimées issues d'un épissage alterne aberrant de pré-ARNm dans des gènes associés à la fonction neurologique normale. La présence de ces variantes d'épissage suggère que des mutations se sont produites dans ces gènes. Des séquences nucléotidiques et des séquence de jonction intron-exon des exemples de variantes d'épissage sont également décrites.

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