A novel modulator of non-genomic activity of nuclear...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) A01K 67/027 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C07K 19/00 (2006.01) C12P 21/02 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2443676

A novel protein modulator of non-genomic activity of nuclear receptors (MNAR), which interacts with estrogen receptor alpha (ER.alpha.), estrogen receptor beta (ER.beta.), and other nuclear receptors, was discovered and characterized. An MNAR-ER complex, in vitro and in-vivo, interacts ands stimulates the enzymatic activity of tyrosine kinases, such as p60c-src (cSRc) and p56lck (LCK). MNAR also stimulates the transcriptional activity of ER. This invention also relates to nucleic acids, antisense, recombinant expression of nucleic acids, as well as host cells, compositions and assays that utilize the nucleic acids and protein of this invention.

L'invention concerne un nouveau modulateur protéique de l'activité non génomique des récepteurs nucléaires MNAR, lequel interagit avec le récepteur oestrogène alpha (ER alpha ), le récepteur oestrogène bêta (ER beta ), et avec d'autres récepteurs nucléaires. L'invention concerne un complexe MNAR-ER, in vitro et in vivo, interagissant avec l'activité enzymatique des tyrosine kinases, telles que p60<c.src> (cSRc) et p56<lck> (LCK) et stimulant cette activité enzymatique. Les récepteurs nucléaires MNAR stimulent également l'activité transcriptionnelle de ER. La présente invention concerne également des acides nucléiques, une expression de recombinaison antisens des acides nucléiques, ainsi que des cellules hôtes, des compositions et des dosages biologiques utilisant ces acides nucléiques et la protéine décrits dans cette invention.

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