C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) A01H 1/00 (2006.01) C12N 9/26 (2006.01)
Patent
CA 2336249
The invention provides a novel chloroplast targeted novel 3-amylase sequence (ct .beta.-amylase), a novel chloroplast targeting nucleic acid sequence and a novel .beta.-amylase sequence. There is also disclosed an inducible promoter which is independently stimulated by light or sugar stimulus. Methods of transforming plants using these sequences are described, as well as transformed plant cells, transformed plants and seed thereof, as well as chimaeric genes containing the sequences. Modification of starch levels in plants can be achieved, as well as the targeting of genes from the starch biosynthetic or degradative pathways, disease or pest resistance or variation of gene expression due to stimulus are described.
La présente invention concerne une nouvelle séquence de .beta.-amylase (ct .beta.-amylase) ciblant un nouveau chloroplaste, un nouveau chloroplaste ciblant une séquence d'acide nucléique, et une nouvelle séquence de .beta.-amylase. Cette invention concerne également un promoteur inductible stimulé individuellement par des stimuli tels que la lumière ou le sucre. En outre, cette invention concerne des procédés de transformation de plantes utilisant ces séquences, des cellules végétales transformées, des plantes transformées et leurs graines, ainsi que des gènes chimères comprenant ces séquences. Par ailleurs, cette invention permet d'effectuer la modification des taux d'amidon chez les plantes, le ciblage de gènes des voies de dégradation ou d'amidon biosynthétique, la résistance aux maladies ou aux parasites, ou encore la variation de l'expression génique en raison des stimuli.
Kavanagh Thomas Anthony
Lao Nga Thi
Advanced Technologies (cambridge) Limited
Fetherstonhaugh & Co.
LandOfFree
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