A novel root specific promoter driving the expression of a...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 5/10 (2006.01) C12N 9/12 (2006.01) C12N 15/29 (2006.01) G01N 33/53 (2006.01) G01N 33/542 (2006.01)

Patent

CA 2430617

The present invention relates to the field of plant molecular biology, more particularly to the root-specific gene expression in plants. The invention provides nucleic acids for a novel transcriptional regulatory root-specific promoter and nucleic acid and protein sequences coding for a new LRR receptor- kinase protein, further specified as a root clavata 1 homolog (RCH1). Further provided are compositions comprising nucleic acids, polypeptides, antibodies and vectors. The invention further provides for methods for modifying cell fate and/or plant development and/or plant morphology and/or plant biochemistry and/or plant physiology comprising the modification of expression in particular cells, tissues or organs of a plant of the novel LRR receptor- like kinase or comprising the expressing of a gene of interest under the control of the novel transcriptional regulatory root-specific promoter. Further are provided compounds interacting with the new polypeptides for use as herbicides or growth regulators.

L'invention concerne le domaine de la biologie moléculaire végétale et plus particulièrement l'expression de gènes spécifiques des racines. L'invention concerne notamment des acides nucléiques pour un nouveau promoteur et un nouvel acide nucléique régulateurs transcriptionnels spécifiques des racines et des séquences de protéines codant pour une nouvelle protéine kinase de type récepteur du domaine LRR (à répétitions riches en leucines), spécifiée comme RCH1 (root clavata 1 homolog). L'invention concerne également des compositions contenant des acides nucléiques, des polypeptides, des anticorps et des vecteurs. L'invention concerne en outre des méthodes permettant de modifier le sort des cellules et/ou le développement de la plante et/ou la morphologie de la plante et/ou la biochimie de la plante et/ou la physiologie de la plante en modifiant l'expression dans des cellules, tissus ou organes particuliers, d'une plante de la nouvelle kinase de type récepteur du domaine LRR ou en exprimant un gène d'intérêt sous le contrôle du nouveau promoteur régulateur transcriptionnel spécifique des racines. L'invention concerne enfin des composés interagissant avec les nouveaux polypeptides destinés à être utilisés comme herbicides ou régulateurs de la croissance.

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