A nucleic acid construct encoding a processing component...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/62 (2006.01) A61K 31/713 (2006.01) A61K 39/39 (2006.01) C07K 14/02 (2006.01) C07K 14/08 (2006.01)

Patent

CA 2407036

A method for enhancing an immune response to a nucleic acid vaccine comprising administering to an animal a nucleic acid construct encoding a fusion protein comprising a processing component and an antigenic polypeptide of interest wherein said processing component provides heterogeneous processing of the antigenic polypeptide when the nucleic acid construct is expressed in a host cell and a resulting enhancement of the immune response. The processing component is derived from an N-terminal portion of PORF2 of Hepatitis E virus.

L'invention concerne une méthode d'amélioration de la réponse immune à un vaccin à acide nucléique consistant à administrer à un animal une construction d'acide nucléique codant une protéine de fusion comprenant un constituant de traitement ainsi qu'un polypeptide antigénique d'intérêt, ledit constituant de traitement procurant un traitement hétérogène du polypeptide antigénique lorsque la construction d'acide nucléique est exprimée dans une cellule hôte et par voie de conséquence une amélioration de la réponse immune. Le constituant de traitement est dérivé d'une partie N-terminale de PORF2 du virus de l'hépatite E.

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