A one-step assay for high-density lipoprotein cholesterol

Details A one-step assay for high-density lipoprotein cholesterol A one-step assay for high-density lipoprotein cholesterol

C - Chemistry, Metallurgy

12

Q

C12Q 1/60 (2006.01) B01D 39/00 (2006.01) G01N 33/52 (2006.01) G01N 33/543 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2474952

Disclosed is a method for quantifying cholesterol in high-density lipoproteins in a single step assay using a dry slide. The method for quantifying cholesterol in high-density lipoprotein comprises a first step of adding a sample onto a multi-layered dry slide wherein at least one of the layers contains phosphotungstic acid and another contains a high-density lipoprotein selective surfactant. The phosphotungstic acid precipitates non-high-density lipoproteins while the high-density lipoprotein selective surfactant only solubilizes high-density lipoproteins and does not solubilize non-HDL precipitated complexes. The cholesterol esterase then reacts with the solubilized HDL cholesterol esters to form cholesterol. Finally the cholesterol in the high-density lipoprotein is detected and quantified.

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