A pathogen and stress-responsive promoter for gene expression

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/54 (2006.01) A01H 5/00 (2006.01) C12N 9/10 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2309808

Nucleic acid sequences and methods for their use are provided for obtaining plants with inducible phenotypes, including novel or enhanced resistance to infection and stress. Novel nucleic acid constructs are provided, which include a transcriptional initiation region from a pathogen inducible-rapid response grape stilbene synthase gene, a DNA sequence of interest and a transcriptional termination region. The constructs are used to prepare expression cassettes which may then be introduced into plants and portions thereof for modulation of expression of endogenous products as well as production of exogenous products in the plant.

L'invention concerne des séquences nucléotidiques et leurs procédés d'utilisation pour obtenir des plantes à phénotypes inductibles, notamment des plantes présentant une nouvelle résistance ou une résistance améliorée à l'infection et au stress. L'invention concerne de nouveaux acides nucléiques recombinés présentant une région de début de transcription issue d'un gène de stilbène synthase de vigne à réponse rapide inductible par un pathogène, une séquence d'ADN d'intérêt et une région de fin de transcription. Ces produit de recombinaison sont utilisés pour préparer des cassettes d'expression que l'on peut ensuite introduire dans des plantes ou dans des parties de plantes en vue de la modulation de l'expression de produits endogènes et de la production de produits exogènes dans la plante.

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