C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
C12M 3/00 (2006.01) B01L 3/00 (2006.01) B01L 3/02 (2006.01) C12M 1/12 (2006.01) C12M 1/36 (2006.01) C12N 5/02 (2006.01)
Patent
CA 2448358
A multiple well plate and method for media exchange, including a body defining a plurality of cell wells each connected via a channel to one of a plurality of aspiration holes, is provided. The cell wells contain a porous, hydrophilic frit which is suspended on a ledge above a reservoir of fluid media and supports a tissue sample. The properties of the frit wick the fluid media upwards to supply the tissue sample with nutrients for growth and proliferation. Old media is aspirated from the wells by a liquid handling device which inserts a pipette tip into the aspiration holes. The pipette tip applies a suction pressure which draws the media out of the cell well, through the channel, into the aspiration hole and out through the pipette tip. New media is dispersed through the pipette tip and directly into the cell well.
L'invention concerne une plaque à multiples cupules et un procédé d'échange de média comportant un corps définissant plusieurs cupules. Chaque cupule est connecté par un canal à l'un des orifices d'aspiration. Les cupules contiennent un disque fritté hydrophile et poreux suspendu sur un rebord placé au-dessus d'un média fluide et qui soutient un échantillon de tissu. Les propriétés du disque fritté drainent le média fluide vers le haut afin d'apporter des nutriments à l'échantillon de tissu pour lui permettre de se développer et de proliférer. L'ancien média est alors aspiré par les cupules au moyen d'un appareil de manipulation de liquides qui insère l'embout d'une pipette dans les orifices d'aspiration. L'embout d'une pipette applique une pression d'aspiration qui aspire le média hors de la cupule, par le canal dans l'orifice d'aspiration et en dehors par l'embout de la pipette. On disperse alors le nouveau média par l'embout de la pipette et on le fait passer directement dans la cupule.
Biolex Inc.
Biolex Therapeutics Inc.
Smart & Biggar
LandOfFree
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