A polynucleotide and polypeptide sequence and methods thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/17 (2006.01) C07K 1/107 (2006.01) C07K 1/18 (2006.01) C07K 1/20 (2006.01) C07K 1/34 (2006.01) C07K 1/36 (2006.01) C07K 14/62 (2006.01) C12N 15/81 (2006.01)

Patent

CA 2755300

The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably Pichia pastoris. The present disclosure also relates to methods of downstream processing for obtaining protein precursor molecules and subsequent conversion of precursor molecules to functional proteins.

La présente invention concerne le domaine des produits thérapeutiques d'ADN recombinant. Elle met en jeu la conception de moyens bio-informatiques, la synthèse d'un gène artificiel pour un précurseur de l'insuline humaine, comprenant une séquence codante de peptide leader, le clonage dans un vecteur d'expression et l'expression dans un organisme, de préférence Pichia pastoris. La présente invention porte également sur des procédés de traitement en aval pour obtenir des molécules précurseurs de protéines et une conversion ultérieure de molécules de précurseurs en protéines fonctionnelles.

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