A polynucleotide comprising a ubiquitous chromatin opening...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/85 (2006.01) A01K 67/027 (2006.01) A61K 48/00 (2006.01) C12N 5/10 (2006.01) C12N 15/00 (2006.01) C12N 15/11 (2006.01) C12N 15/86 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2333852

The present invention relates to a polynucleotide comprising a ubiquitous chromatin opening element (UCOE) which is not derived from an LCR. The present invention also relates to a vector comprising the polynucleotide sequence, a host cell comprising the vector, use of the polynucleotide, vector or host cell in therapy and in an assay, and a method of identifying UCOEs. The UCOE opens chromatin or maintains chromatin in an open state and facilitates reproducible expression of an operably-linked gene in cells of at least two different tissue types.

La présente invention se rapporte à un polynucléotide comportant un élément d'ouverture de la chromatine ubiquiste (UCOE ubiquitous chromatin opening element) qui n'est pas dérivé d'une région LCR. La présente invention se rapporte également à un vecteur comportant la séquence nucléotidique, à une cellule hôte comportant ledit vecteur, à l'utilisation dudit polynucléotide, dudit vecteur ou de ladite cellule hôte à des fins thérapeutiques ou d'analyse, et à une méthode d'identification de ces UCOE. Un UCOE ouvre la chromatine ou maintient la chromatine dans un état ouvert et facilite l'expression reproductible d'un gène lié fonctionnellement dans des cellules d'au moins deux types de tissus différents.

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