A process for distinguishing nucleic acids on the basis of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2078132

A process is provided for distinguishing nucleic acids on the basis of nucleotide differences in random segments of the nucleic acid by performing a primer extension reaction on the nucleic acids and comparing the extension reaction products. The random nucleic acid segment can be amplified by first dissociating the extension product from the template and contacting the dissociated extension product with a primer under conditions such that an amplification extension product is synthesized using the dissociated extension product as a template. Differences in the extension product are useful as markers in constructing genetic maps and as markers to distinguish or identify individuals.

Procédé de distinction d'acides nucléiques sur la base de différences de nucléotides dans des segments aléatoires de l'acide nucléique, consistant à procéder à une réaction d'extension d'amorce sur les acides nucléiques et à comparer les produits de la réaction d'extension. On peut amplifier le segment d'acide nucléique aléatoire, premièrement en dissociant le produit d'extension du gabarit et en mettant en contact le produit d'extension dissocié avec une amorce dans des conditions telles qu'un produit d'extension d'amplification est synthétisé à l'aide du produit d'extension dissocié en tant que gabarit. Les différences dans le produit d'extension sont utiles en tant que marqueurs dans l'élaboration de cartes génétiques et en tant que marqueurs permettant de distinguer ou d'identifier des individus.

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