C - Chemistry – Metallurgy – 08 – B
Patent
C - Chemistry, Metallurgy
08
B
C08B 31/00 (2006.01) C08B 30/12 (2006.01) C12N 9/28 (2006.01) C12P 19/04 (2006.01) C12P 19/14 (2006.01)
Patent
CA 2415521
An alpha-amylase enzyme obtained from Bacillus acidocaldarius species is utilized to liquefy starch at a pH as low as 3.0 without the need to add thermostabilizing agents such as calcium. The alpha-amylase produces acceptable DE yields in a single liquefaction step and does not need to be inactivated prior to conducting saccharification which can proceed without adjustment of the pH of the liquefact. Alternatively, a secondary liquefaction process can be utilized, in which case two additions of the alpha-amylase are used resulting in a combined low dosage of the enzyme.
Une alpha-amylase obtenue à partir d'espèces de Bacillus acidocaldarius est utilisée pour hydrolyser de l'amidon à un pH aussi bas que 3,0 sans avoir à ajouter d'agents thermostabilisants comme du calcium. L'alpha-amylase produit des rendements en DE acceptables en une seule étape d'hydrolyse et n'a pas besoin d'être inactivée avant de réaliser la saccharification qui peut avoir lieu sans ajustement du pH. Autrement, un deuxième procédé d'hydrolyse peut être utilisé, au cours duquel on fait deux ajouts d'alpha-amylase conduisant à un faible dosage combiné de l'enzyme.
Shetty Jayarama K.
Singley Eric C.
Strohm Bruce A.
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Genencor International Inc.
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