A process for immobilizing cells on a resin

C - Chemistry – Metallurgy – 12 – P

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C12P 19/38 (2006.01) C12N 1/20 (2006.01) C12N 9/10 (2006.01) C12N 11/00 (2006.01) C12N 11/02 (2006.01) C12N 15/54 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2581848

A process for immobilizing cells is described, particularly procaryotic cells, which includes a step of adsorbing said cells on a resin, wherein such a resin is a weak anionic exchange resin an preferably has amino moieties; when the immobilized cells are UPase and/or PNPase producing cells, nucleosides can be produced by reaction of a pentose-1-phosphate with a purine or pyrimidine base, and transglycosylation reactions can be performed; advantageously, as immobilized UPase or PNPase producing cells , Escherichia cells of the DH5alpha strain transformed by the plasmid vectors having the sequences reported in Seq. Id. No. 1 and 2 are used.

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